To validate the reliability of the transcriptome sequencing data, the relative expression levels of 9 DEGs involved in Alzheimer’s disease (Apoe, Ptgs2, Rest, Zbed6, Loxl2, Ccl2, Synpo, Ablim1, and Glis3; see also Table 2) were analyzed by quantitative RT-qPCR. The gene discussed is GLIS3; the disease is early-onset autosomal dominant Alzheimer disease.