PRKN and neoplasm: Conversely, reconstitution of Parkin-expressing cells with K2-DM or K2 mutants K581A or K582A restored tumor cell adhesion (Fig. S5A) and spreading (Fig. S5, B and C), rescued single-cell movements (Fig. 3, A and B), and enabled tumor cell invasion across Matrigel inserts (Fig. 3, C and D), as well as in 3D tumor spheroids (Fig. 3, E and F).