To examine the functions of HPK1 in KrasG12D-mediated formation of PanINs and their progression to invasive PDAC, KrasG12D Bac-cre (KC), KrasG12D HPK1 Bac-cre (KHC), and KrasG12D M46 Bac-cre (KMC) mice were treated with tamoxifen followed by ICer treatment — which accelerate the development and progression of PanINs — for 2 days to induce pancreatitis. This evidence concerns the gene CALCA and pancreatitis.