Statistical analysis showed that the positive expression of SMO in benign mesothelial tissue and malignant mesothelioma was 14.00% (7/50) and 81.54% (106/130) of biopsy specimens, respectively, with statistically significant differences (P < 0.05, Table 5); GLI1 positivity was 18.00% (9/50) and 76.15% (99/130), respectively. This evidence concerns the gene SMO and malignant mesothelioma.