Using X to denote the expression fold of mesothelioma tissue compared to benign mesothelial tissue, then X = 2ΔΔCt, when X ≥ 2, ΔΔCt ≥ 1, indicating that the expression of target gene (SMO and GLI1) in malignant mesothelioma tissue is higher than that in benign mesothelial tissue; when X < 2, ΔΔCt < 1, indicating that the expression of target gene (SMO, GLI1) in malignant mesothelioma tissue is the expression in malignant mesothelioma tissues was lower than that in benign mesothelial tissues. Here, GLI1 is linked to malignant mesothelioma.