GLI1 and mesothelioma: Using X to denote the expression fold of mesothelioma tissue compared to benign mesothelial tissue, then X = 2ΔΔCt, when X ≥ 2, ΔΔCt ≥ 1, indicating that the expression of target gene (SMO and GLI1) in malignant mesothelioma tissue is higher than that in benign mesothelial tissue; when X < 2, ΔΔCt < 1, indicating that the expression of target gene (SMO, GLI1) in malignant mesothelioma tissue is the expression in malignant mesothelioma tissues was lower than that in benign mesothelial tissues.