After screening for confounding factors based on the results of univariate analysis and clinical significance (p < 0.1, i.e., univariate significance), age at delivery, pre-pregnancy BMI, net weight per week, ALT, ALB, GGT, AST, LDH, WBC, NE, TG, MO, G, P, HBV infection, GDM, thalassemia, hyperthyroidism, hypothyroidism, uterine malformation were gradually incorporated into the model. The gene discussed is GPT; the disease is hypothyroidism.