Next, CD31 immunohistochemistry staining results revealed that the vascular morphology of the tumor was intact and clearly visible before LIFU-TMD treatment, while LIFU-TMD mediated damage to tumor vascular endothelium and structure was confirmed by the unevenly staining and invisible tubular architectures after LIFU irradiation (Figure 3C). This evidence concerns the gene PECAM1 and neoplasm.