VIP and Aganglionic megacolon: It was higher in submucosal and myenteric plexus neurons in the dilated portion of the megacolon compared to the undilated portion and the uninfected population, and this might be related to the preferential destruction of inhibitory motor neurons (VIP and NOS immunoreactivity) in the intestine of chagasic patients with megacolon by Trypanosoma cruzi and inflammatory processes (30).