ACOX1 and leukodystrophy: While fully aware of the limitations of the model, we decided to use the BV-2 cell line and targeted the Abcd1 and Abcd2 genes, which encode for partially redundant peroxisomal ABC transporters of CoA esters of VLCFAs (Mosser et al., 1993; Lombard-Platet et al., 1996; Genin et al., 2011; Tawbeh et al., 2021), and the Acyl-CoA oxidase 1 (Acox1) gene, which controls the first step of peroxisomal β-oxidation and whose defect is associated with a rare leukodystrophy (MIM 264470; Fournier et al., 1994; Nohammer et al., 2000; Ferdinandusse et al., 2007; Vamecq et al., 2018).