However, our results differ from studies in breast cancer cells (Peng et al., 2019) or the effects of hyperosmotic stress in keratinocytes (Silva et al., 2015), in which ROCK increased phospho-cofilin (i.e., inactivation), or studies in which ouabain inhibits Na+K+-ATPase activity by decreasing ROCK activation, which resulted in cofilin activation (Jung et al., 2006). The gene discussed is CFL1; the disease is breast cancer.