Meanwhile, it was indicated that about 64.35% of the FG-labeled neurons were activated by itch stimuli, and about 82.03% of the itch-activated CM-projecting neurons in the PBN were VGluT2-positive neurons (supplementary Figs. 3d, e), indicative of the glutamatergic projections from the PBN to the CM in itch information transmission. The gene discussed is SLC17A6; the disease is Pruritus.