A point mutation in the kinase domain of LRRK2 (LRRK2-p.G2019S) was previously determined to disrupt the axonal transport of AVs in NGN2-induced human neurons (iNeurons) that express endogenous LRRK2.10,24,25 Mutations in the p.R1441 hotspot located in the ROC domain of LRRK2 also cause PD, potentially with higher penetrance.5,26,27 Consistent with previous reports, we found that LRRK2-p.R1441C causes a higher magnitude of kinase hyperactivity than LRRK2-p.G2019S in KI mouse embryonic fibroblasts (MEFs, Figure S1). Here, LRRK2 is linked to Parkinson disease.