We first infused 10,000 (effector:tumor = 1:1) of second generation (2nd-gen) anti-CD19 4-1BBζ-CAR T cells (hereafter CAR T cell, unless stated otherwise) per chip into the perfusable vessels from central sinus and quantified the count of pre-seeded leukemia blasts on-chip (GFP-expressing Reh, unless stated otherwise) daily for over 7 days. This evidence concerns the gene CD19 and leukemia.