To exclude the influence of endogenous TMEM134 protein, we generated a Tmem134 knockout Myc AML monoclonal cell line (i.e., M#8) with CRISPR/Cas9 and then introduced TMEM134α or TMEM134β cDNA into M8# by retrovirus (S9C–S9G Fig). The gene discussed is TMEM134; the disease is acute myeloid leukemia.