During leukemogenesis, we harvested the initiating Myc-overexpressing HSPCs (pretransplanted, T0), preleukemic BM cells at 2 weeks (T1), 4 weeks (T2) after transplantation, and full-blown AML BM cells (T3, 8 weeks after transplantation) for 10× Genomics scRNA-seq analyses, and a total of 41,078 cells were retained for analysis. Here, MYC is linked to acute myeloid leukemia.