TGFB1 and diabetes mellitus: Therefore, methods to increase TGF-β such as surgical debridement, which utilizes the principle that exposure to fresh serum, as occurs when a fresh wound is inflicted, bathes the skin in TGF to promote re-epithelialization or the use of nitric oxide that activates the latent form of TGF-β may be used to aid DM wound healing [1,19]. Components in cold atmospheric plasma such as growth factors, like FGF-2 and VEGF, and interleukins mediate granulation, vascularization and reepithelization in the process of healing in a diabetic foot [20].