Ten HLA-I bound peptides derived from the melanoma-associated gene family sources MAGE-A1 and MAGE-A4, which are known to be expressed in many tumor types but not in normal tissues except for testis and placenta, were expressed and presented mainly in the CD3+CD8+ T cell-excluded LUSC tumors (that is, 02288 and 02289), supporting a previous study showing an association of MAGE-A4 expression in LUSCs compared with LUADs30. This evidence concerns the gene MAGEA4 and melanoma.