Therefore, we determined the CD3+CD8+ T cell infiltration after pathological inspection with hematoxylin and eosin staining and mIF staining of T cell tumor infiltration markers (CD3, CD8, granzyme B (GrzB), Ki67, cytokeratin (CK) and DAPI) (Fig. 3a,b and Extended Data Fig. 2) in one randomly selected macro-region tissue per patient. The gene discussed is MKI67; the disease is neoplasm.