AKT1 and obesity due to melanocortin 4 receptor deficiency: Therefore, monitoring dynamics of the AKT pathway could provide detailed insights in BAT thermogenesis in response to various interventions or treatments and the Akt-FRET biosensor mouse described here will be a useful tool to monitor therapeutic efficacies of potential anti-obesity medications by monitoring dynamic changes of the AKT pathway within a live physiologically relevant microenvironment.