PGK1 and infection: The quantitative real-time PCR (qRT-PCR) analysis of PK-15 cells infected with Heat-SVA and SVA at MOIs of 0.1 or 1 for 48 h showed that SVA infection and replication induced dose-dependent increases in the expression of HK2, PFKM, PKM, PGK1, HIF-1α, and SOD2, but not in Heat-SVA-treated or uninfected cells (Fig 1C).