In vivo, prostate cancer cells from PC3 lineage cells with T. vaginalis-contained medium (TCM) injected into a PC3 xenograft mouse model resulted in an increase in tumor weight and volume and in EMT-related markers, proliferating cell nuclear antigen (PCNA) and cyclin D1, which are proliferative signal molecules [64]. Here, PCNA is linked to prostate carcinoma.