Subsequent molecular experiments showed that TRIM9 suppression was associated with the enhanced mesenchymal phenotypic transition of tumor cells, since the mRNA and protein expression of mesenchymal-related cell biomarkers (N-cadherin and vimentin) was significantly increased in the TRIM9 shRNA-treated KYSE-410 cell group, while the expression of an epithelial-related biomarker (E-cadherin) was suppressed (Figures 2(h) and 2(i)). Here, TRIM9 is linked to neoplasm.