At end point using the E0771 orthotopic murine breast tumor model (1.2 cm), differential gene expression by RNA-sequencing of wild-type versus HIF-2α-deficient TAMs demonstrated 3 genes: Spint1, IL-10, and Depdc7 were downregulated by HIF-2α knockout and had an identified HIF-2α (not HIF-1α) binding site using previously published ChIP-seq data sets [47]. This evidence concerns the gene IL10 and breast neoplasm.