To investigate the effects of Nrf2 on macrophages and its role in AOM solution, we generated an adeno-associated virus (serotype 6) containing Nrf2 under the macrophage-specific CD11b promoter (AAV-pCD11b-Nrf2) and a control AAV containing a scramble sequence (Scr) under the CD11b promoter (AAV-pCD11b-Scr). The gene discussed is ITGAM; the disease is infectious otitis media.