Our findings corroborate with previous studies where it was reported that high glucose concentrations (15mM, 25mM) do not alter the expression of TNF-α and IL-6 by macrophages, however, when stimulated with LPS, the secretion of these cytokines increase, showing that hyperglycemia it is not a sufficient stimulus for the high production of these cytokines by macrophages (35, 38). Here, TNF is linked to Hyperglycemia.