Among most of these tumor types, a negative correlation between gene methylation and mRNA expression was revealed by calculating the Spearman correlation, including HIF1A, SLC16A1, UEVLD, SLC16A8, PFKFB2, LDHB, and SLC16A3 (Supplementary Figures 1A, B). This evidence concerns the gene PFKFB2 and neoplasm.