For instance, accumulation of mutant huntingtin in cells treated with autophagy inhibitors such as the lysosomal proteolysis disruptors ammonium chloride and leupeptin and the autophagy induction inhibitor 3-MA can be monitored using immunofluorescence in both cells isolated from HD patients and mouse cells (Koga et al., 2011). This evidence concerns the gene HTT and Huntington disease.