In this depression model, cells were engineered to knockout FKBP51 and Akt1/2 and treated with different antidepressants, including paroxetine, amitriptyline, and fluoxetine, and autophagy induction was assessed by identifying autophagy markers and autophagic flux by monitoring the LC3 fluorescence signal (Gassen et al., 2014). Here, AKT1 is linked to major depressive disorder.