By applying traditional (e.g., Western blot) and more sophisticated analytical techniques such as gene reporter assays (e.g., DsRed-T3 or mCherry-GFP-LC3B reporter assays), key autophagy players (e.g., LC3I, LC3II, P62, LAMP2A, and Hsc70) can be monitored to assess aberrant autophagy and the effect of the presence of mutant proteins relevant to ALS (Perera et al., 2017). This evidence concerns the gene SQSTM1 and amyotrophic lateral sclerosis.