A: Identification of macroautophagy regulation by neuronal activity after neuronal stimulation by chemical long-term depression.D: Cells were treated with the autophagy inhibitor BafA1 in chemical long-term depression conditions in combination with chemical long-term depression inhibitors. Cells were engineered to knock down the expression of Atg7 and to express EGFP-LC3B.R: (1) Protein expression by Western blot targeting LC3, Akt, p-Akt, mTOR, p-mTOR, and Atg7. (2) Quantitation of autophagosomes and lysosomes and (4) cell morphology were conducted by confocal microscopy targeting LC3. This evidence concerns the gene ATG7 and major depressive disorder.