MAP1LC3A and Huntington disease: In HD studies focusing on macroautophagy, autophagic flux is monitored using different techniques such as protein expression by Western blot targeting LC3II and LC3I, immunostaining of LC3, or using the novel tandem fluorescent-tagged mCherry-GFP-LC3 system (Proenca et al., 2013; Ehrnhoefer et al., 2018).