In this depression model, cells were engineered to knockout FKBP51 and Akt1/2 and treated with different antidepressants, including paroxetine, amitriptyline, and fluoxetine, and autophagy induction was assessed by identifying autophagy markers and autophagic flux by monitoring the LC3 fluorescence signal (Gassen et al., 2014). This evidence concerns the gene MAP1LC3A and depressive symptom measurement.