Finally, we tested all the candidate genes in the AD mice model to verify the biomarkeEPS8, ZMIZ1, TELO2, PRKCZ, and Man1b1, although the number of these genes is significantly less than that of candidate genes (possibly related to species differences), this has partly suggested that the genes we screened may be universal in the biological world, so these five genes can be well-verified. This evidence concerns the gene MAN1B1 and Alzheimer disease.