SIM2 and breast cancer: To further determine the effect of SIM2s on metabolism, we generated CRISPR/Cas9 SIM2 knock-out (SIM2KO) cells (Supplementary Fig. 1c), which also underwent an EMT (Supplementary Fig. 1d–g) and overexpressed FLAG-tag labeled SIM2s (SIM2s-FLAG) in SUM159 breast cancer cells that did not express SIM2s43,44,48.