Notably, all four samples with CDC73 two-hit mutations (P5, P6, P11, and P22) showed elevated intratumor variant allele frequency (VAFtumor) of somatic variants, while the VAFtumor of germline variants decreased in tumor DNA, suggesting copy number gains in CDC73Som and/or losses in CDC73Germ (Fig. 4a, Supplementary Fig. 9, see Methods). This evidence concerns the gene CDC73 and neoplasm.