Moreover, Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis indicated that these variable genes are particularly associated with several specific intracellular pathways including Nrf2-mediated oxidative stress response, relevant to external hyperthermia stimulus35, p53 signaling36, regulation of epithelial-mesenchymal transition (EMT) pathway and inhibition of matrix metalloproteases that are closely participated in tumor migration37,38, suggesting the effectiveness of TRPV1 blockade to potently regulate cell apoptosis and potentially suppress tumor metastasis (Fig. 2e). This evidence concerns the gene TP53 and neoplasm.