Via applying the A549-TRPV1 knockdown (A549-TRPV1 KD) tumor model and transcriptome analysis, TRPV1 blockade is found to specifically block calcium influx upon hyperthermia at tumor, and results in distinct inhibition of HSF1 nuclear translocation for selectively suppressing stressfully overexpressed HSP70 to reverse thermo-resistance. This evidence concerns the gene HSF1 and neoplasm.