To demonstrate that TRPV1 blockade might enable the manipulation of tumor stroma via suppressing HSF1-mediated TGFβ1 upregulation, we thus firstly investigated the influence of TRPV1 blockade on HSF1 distribution in the PANC02 tumor cells upon hyperthermia using immunofluorescence staining. This evidence concerns the gene TRPV1 and neoplasm.