To explore the in vivo synergy of TRPV1 blockade with thermotherapy, IS-Micelles were administrated into the orthotopic 4T1-Luc breast tumors-bearing mice at a single dose of 7.5 mg kg−1 ICG and 5.0 mg kg−1 SB705498, followed by light exposure (785 nm, 1.5 W cm−2) for 5 min and subsequent measurements of tumor volumes during 21 days post-injection. Here, TRPV1 is linked to breast neoplasm.