PBRM1 and nonpapillary renal cell carcinoma: Third, we did not sequence each subregion within the tumor for loss of function mutations such as VHL, BAP1, and PBRM1. Given that VHL mutation is the only consistent truncal mutation in ccRCC, and nearly two-thirds of other ccRCC driver mutations are subclonal, a single region, single cell approach would almost certainly miss substantial ITH present at any level [1].