To visually phenotype clusters 2, 4, 6, 11, and 19, we mapped the single-cell average intensities of L-ferritin, HLA-DR, CD68, CD74, and Iba1 on merged t-SNEs (all microglia from ALS and control motor cortex and hippocampus) as a binary high-low approach to visualise MOIlow and MOIhigh microglia (Fig. 5G). This evidence concerns the gene CD68 and amyotrophic lateral sclerosis.