To further investigate the role of USP37 in cellular survival under replication stress in osteosarcoma cells, we inhibited USP37 using si-USP37 and exposed U2OS osteosarcoma cell line to HU-mediated replication stress (100 μM, 200 μM, 300 μM HU) for 24 h. Here, USP37 is linked to osteosarcoma.