In order to validate our finding further, we treated cells that overexpressed USP37 with HU and immuno-stained osteosarcoma cells with Anti USP37 antibody as well as Anti 53BP1 and Anti Replication protein A antibody to assess to resolution of these DNA damage foci in cells that were over expressing USP37 as compared to cells in which its expression was low. The gene discussed is TP53BP1; the disease is osteosarcoma.