We determined apoptosis related proteins (including Bad, bcl2, Bax and cleaved Caspase3) and cell cycle-related proteins (including p21, Cyclin D1, CDK4, Cyclin E1, and CDK2) levels in HCC cells, and found that SATB2-AS1 promoted Bad, Bax, cleaved Caspase3, and p21 levels in HCC cells, but reduced bcl2, Cyclin D1, CDK4, Cyclin E1, CDK2 expressions. This evidence concerns the gene CCND1 and hepatocellular carcinoma.