Linear discriminant analysis effect size analysis further revealed that abundances of major discriminators, such as Paraprevotellaceae, Prevotella, Enterobacteriaceae, and Enterobacteriales, which are well-known pathobionts implicated in irritable bowel syndrome and IBD [43], were significantly higher in WD-fed Tas1r3+/+ mice (Fig. 6j). This evidence concerns the gene TAS1R3 and inflammatory bowel disease.