DE pathway analysis revealed the enrichment of Kyoto Encyclopedia of Gene and Genomes (KEGG) pathways related to general neurodegeneration, AD and other neurodegenerative diseases (Extended Data Fig. 5c,d and Supplementary Table 1), indicating that clusters 7 and 18 represent neuronal APOE4-promoted disease-associated neurons (nE4-DANs). This evidence concerns the gene APOE and neurodegenerative disease.