Two-color immunofluorescence revealed that the majority of Mincle-expressing cells were macrophages as identified by co-expressing CD68 and Mincle (CD68 + Mincle + ) (Fig. 1E), providing a direct evidence for Mincle-expressing macrophages in psoriasis lesions and a rationale for studying the role of Mincle in macrophage-mediated psoriasis. Here, CLEC4E is linked to psoriasis.