In order to determine the effect of Mincle on macrophage-mediated psoriasis injury, one day after IMQ treatment, we injected clodronate liposomes subcutaneously to the mouse skin lesions to deplete the macrophages locally, followed by adoptively transferred via tail vein with Tomato-expressing BMDM (macrophages expressing red fluorescence, Supplementary Fig. 1A) or Mincle-KO BMDM on the 3rd and 5th days after clodronate liposomes administration (Fig. 3A, B). The gene discussed is CLEC4E; the disease is psoriasis.