Given the fact that the expression of TRIM38 is downregulated in NAFLD, we further generated a Trim38 systemic KO (Trim38-KO) (Fig. 2A) mouse strain via CRISPR-Cas9 system to reveal the function of TRIM38 in the pathology of NAFLD process in vivo. Here, TRIM38 is linked to metabolic dysfunction-associated steatotic liver disease.