To explore the biological function of Irg1 during tumor development, we generated different syngeneic tumor models in which B16-F10 (melanoma), MC38 (colon cancer), and E0771 (breast cancer) cells were inoculated subcutaneously into Irg1−/− and Irg1+/+ mice. This evidence concerns the gene ACOD1 and malignant colon neoplasm.