Among the population of MФ and monocytes, Vcan+ monocytes exhibited up-regulation of IFN-stimulated genes (e.g., Irf7, Isg15, Ifi44, and Ifi27l2a) in the TME of B16-F10 tumors from Irg1−/− mice compared with Irg1+/+ controls, and genes associated with antigen presentation and T cell migration (e.g., Aif1, Timd4, and Tap1) were also up-regulated in proinflammatory macrophages from tumor-bearing Irg1−/− mice (Fig. 3B). Here, ISG15 is linked to neoplasm.