Whereas basal cardiac function did not differ between WT and Isg15–/– mouse hearts, following 20 minutes of ischemia and 40 minutes of reperfusion, left ventricular developed pressure (LVDP) (Figure 10A), recovery of LVDP (Figure 10B), and dP/dtmax (Figure 10C) were improved in Isg15–/– mouse hearts in comparison with WT mouse hearts, with no difference observed in dP/dtmin or heart rate (Figure 10, D and E). Here, ISG15 is linked to ischemia.