Results indicated that exposure to 42°C for 1 hour induced a significant concomitant reduction of PD-L1 and NLRC5 expression in ovarian cancer cell lines cocultured with PBMC at both mRNA (PD-L1: 0.2824 ± 0.041-fold change, 0.77 ± 0.044-fold change, NLRC5: 0.46 ± 0.08-fold change, 0.8967 ± 0.01098 in IGROV1 and SKOV3 respectively) as well as the protein levels (Figure 2a and b). Here, CD274 is linked to ovarian carcinoma.