As the latter increased when conditioned media from heat-shocked ovarian cancer cells was added to non-treated cells, and since our previous study of HSPs kinetics showed that mainly HSP27 and HSP70 accumulate in the media during the first 24 hours post heat shock, HSPB1 and HSPA1A genes were knocked down in cells from which conditioned media is collected. Here, HSPA1A is linked to ovarian cancer.