The IHC staining of E-Cad, MMP2, and PCNA revealed that in 3d-ACS, the epithelial-mesenchymal transition (EMT), migration, anti-apoptosis, and the malignant phenotype of GC cells were similar to that of 2d xenografts in vivo (Figures 5B–G). The gene discussed is CDH1; the disease is gastric cancer.