These results demonstrated that the NS3 (K107R) and NS5 (I563V) viruses were undetectable over days 1–2 and days 1–3 post-infection in mosquito C6/36 cells and Vero cells, respectively, and they have lower viral titers at early replication when compared to the NS2A (K81R) and WT viruses. Here, RAF1 is linked to infection.