These data showed that JEV could stimulate the activation of NFκB upstream genes IRF3 and IRF7, and the upstream genes of interferon pathway RIG-1 and TLR7; the caspase family, such as caspase-1 and caspase-9; and other genes significantly increased after infection in normal cells, indicating that the inflammatory responses of cells did become stimulated after JEV infection. This evidence concerns the gene IRF3 and infection.