ZC3HAV1 and infection: The percentage of infected ZAP KO 293T cells is similar for all viruses when infected at a multiplicity of infection (MOI) of 1 plaque forming unit (PFU)/cell for 24 h, but while there is a decrease in infected cells in WT 293T cells compared to ZAP KO, the degree of decrease appears to be greater for RRV compared to the other viruses (Figure 1A).