Furthermore, a cytokine analysis of the serum and tumor samples from the mice bearing B16 melanoma, treated by LIVP-FlaB-RFP, indicated a significant elevation of the cytokines: TNF-α, GM-CSF, CXCL9, IL-4, and, to a lower extent, the elevation of IL-10, CCL3, CXCL10, IFN-α, IFN-γ, VEGF, and CCL2 in samples from the tumors treated by LIVP-FlaB-RFP, while in the serum analysis of this group, a slight increase of only TNF-α, VEGF, and IL-6 was observed. The gene discussed is TNF; the disease is melanoma.