Cytotoxic activity: In vitro cytotoxic activity against human breast cancer cell line (MCF-7)After 48 h of treatment:3: IC50 = 7.0 ± 1.8 μMCurcumin (positive control): IC50 = 19.7 ± 2.5 μMCompound 3 induces ΔΨm loss to release the mitochondrial nucleases in MCF-7 cells.Compound 3 increases intracellular Ca2+ levels and activates calpain in MCF-7 cells.Compound 3 induces LMP to release cathepsin B and activate Bid in MCF-7 cells.Compound 3 arrests the S and G2-M phases in MCF-7 cells. The gene discussed is BID; the disease is breast cancer.