We observed that HE-THD significantly abrogated the expansion and proliferation of Tregs induced by TNF (Figure 1 and Figure 2), while it failed to suppress but paradoxically promoted the differentiation of Th1 cells and Th17 cells upon stimulation with IFN-γ and IL-17, respectively (Supplementary data S1). This evidence concerns the gene TNF and hereditary elliptocytosis.