In cell culture, 293T cells transfected with increasing amounts of IFITM1, IFITM2, or IFITM3 expression plasmids showed a dose-dependent increase in the expression of IFITM protein, which also correlated with the degree of inhibition of infection by a HCoV-229E-spike protein pseudotyped virus particle [23]. This evidence concerns the gene IFITM3 and infection.