To specifically assess the polarization state of microglia/macrophages after stroke, representative M1-like (CD16/32) or M2-like (CD206) marker proteins were analyzed by double immunofluorescent staining with the microglia/macrophage marker Iba1 in the penumbral region of cortex and the striatum of mice after MCAO. This evidence concerns the gene AIF1 and stroke disorder.