It was found that the tumor uptake of [99mTc]Tc-CNMCHDG in S180 tumor-bearing mice were significantly inhibited to 2.64 ± 0.48%ID/g (43%) from 4.66 ± 0.34%ID/g and increased to 6.30 ± 0.56%ID/g (35%) at 60 min post-injection by pre-treatment with D-glucose and insulin, respectively, which was in agreement with the in vitro cell uptake study (Figure 4B). The gene discussed is INS; the disease is neoplasm.