As shown in Figure 5A–C, all four ligands could obviously accumulate in PSMA-positive tumors, among which DOTA-(2P-PEG4)2 exhibited the highest tumor uptake value, maintaining over 20ID%/g, and this value rose to 21.85 ± 3.53ID%/g at 2 h. The gene discussed is FOLH1; the disease is neoplasm.